Project

Part:BBa_K3218008:Design

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-16)


CRISPR-X: deactivated Cas9 coupled to activation induced deaminase (AID)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1992
    Illegal BglII site found at 2451
    Illegal BglII site found at 2540
    Illegal BglII site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 498
    Illegal SapI site found at 599


Design Notes

identifying what directino our gRNA would eb facing in order to target the appropriate locus was required with the design.


Source

MS2 protein comes from bacteriophages SV40 NLS is yeast codon optimized from the simian virus AID is from human lymphocyte B cells dCas9 is part of CRISPR mechanism to defend bacteria against viral attack

References